Errors in the medical report of urine cellular fraction

Abstract

Urine examination guides doctors in formulating diagnosis and therapies or in excluding diseases, not only of the urinary tract.
The research for urine cells has been and still is performed manually with manual optical microscopes.
Recently, in the reading of cells found in the urine, various technologies such as automated microscopy and cytofluorometry are used.
This change involved different preparation of the urine sample, microscope field diversity, sample volume diversity, different units of measurement, diversity of standard values.
This change has not been accompanied by the change of terminology in urine examination reporting, resulting in conceptual errors, communication and interpretation by the clinicians.
The job examines the errors and proposes the necessary changes.

 

Keywords. Error, Urine examination, Microscopy, Cytofluorometry.

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PREMESSA

Con il termine sedimento si intende il deposito lasciato in fondo a un recipiente da sostanze o cellule in sospensione.

Il medico ha, da molti decenni, conoscenza che i valori normali di cellule presenti nel “sedimento delle urine” sono:

  • inferiore o uguale a 1 emazia/campo microscopia manuale (superficie 0.237 mm2) a ingrandimenti totali 400x su campione di 10 ml di urine centrifugate, il cui sedimento è ri-sospeso a 0.5 ml
  • inferiore o uguale a 2 leucociti/campo microscopia manuale (superficie 0.237 mm2); ingrandimenti totali 400x su campione di 10 ml di urine centrifugate, il cui sedimento è ri-sospeso a 0.5 ml [1]

 

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