Application of proteomics has become one of the leading experimental disciplines for increased understanding of the key role played by proteins and protein–protein interactions in all aspects of cell function. There is an increasing use of proteomic technologies for investigation into renal replacement therapy such as hemodialysis. In the last 10 years, the application of shotgun bottom-up liquid chromatography-mass spectrometry/mass spectrometry approaches has been successfully applied to research in uremic toxicity, with the discovery of novel uremic toxins and the potential to delineate a precise molecular approach to defining the biochemical nature of uremia.
Major investigations of proteomics in hemodialysis therapy include molecular definition of uremic toxicity, identification of prognostic biomarkers, blood purification efficiency testing, and biocompatibility assessment of the dialyzer membrane materials.
In this article, we review the results of recent proteomic investigations in the setting of chronic hemodialysis therapy.
KEY WORDS: Proteomic, hemodialysis, membrane, uremic toxins, biocompatibility, adsorption, proteins.